Preliminary Study on Resistance Mechanism of the New Rice Line YSBR1 to Sheath Blight

doi:10.3969/j.issn.1001-7216.2014.02.004

Abstract

Abstract: The preliminary mechanism of the new rice line YSBR1 with high resistance to sheath blight disease(SB) was investigated through analyzing the content of wax and silicon, the arrangement of silicon cell, the activity of the wellknown defense enzymes, and the expression pattern of some pathogenesisrelated (PR) genes in response to SB fungus infection. The wax contents of leaves and leaf sheaths were not significantly different among varieties with various resistance levels to SB disease including YSBR1. The arrangement of silicon cells in the upper and lower surfaces of leaf blades was similar among varieties, and no silicon cell was found in the inner surfaces of leaf sheaths. In response to SB fungus infection, the contents of silicon in the lower surfaces of leaf blades and the inner surfaces of leaf sheaths in YSBR1 were significantly induced, as well as in inner surfaces of leaf sheaths of Jasmine 85 with moderate resistance to SB. However, it was not found in all tissues tested in susceptible Lemont. In YSBR1, the activities of phenylalanine ammonia lyase(PAL ) and polyphenol oxidase(PPO) were induced faster and significantly higher by SB fungus than those in other varieties. The expression level of OsPR4 in YSBR1 was upregulated rapidly and obviously higher than those in Jasmine 85 and Lemont 12 h post inoculation. The OsPR10 gene was found constitutively expressed in YSBR1 and Lemont, but induced expression in Jasmine 85 by SB fungus infection. Taken together, our results suggest that the defense system of YSBR1 is rapidly activated upon SB fungus infection,which is responsible for its high resistance to SB.

Key words: rice sheath blight, resistant variety, wax content, silicon, defense response

摘要： 通过测定蜡质含量、硅化细胞、硅含量以及相关防御酶和防卫基因诱导表达特征，对抗纹枯病品系YSBR1的抗性机制进行了初步研究。结果显示，叶片和叶鞘的蜡质含量在不同抗、感品种间差异不显著；叶片上、下表面的硅化细胞排列密度在各品种间总体差异不明显，各品种叶鞘内侧均未发现硅化细胞；纹枯菌接种后YSBR1叶片下表面和叶鞘内表面硅元素含量均显著或极显著上升，中抗品种Jasmine 85叶鞘内表面硅含量亦极显著上升，感病品种Lemont不同部位的硅含量在处理之间差异均不显著；纹枯病菌接种后YSBR1中的苯丙氨酸解氨酶和多酚氧化酶活性的诱导表达及活性高峰值总体上快于或高于其它供试品种；接种后12 h，防卫基因OsPR4的表达量在YSBR1中急速上升且明显高于Jasmine 85和Lemont，OsPR10在特青中为诱导表达，但在YSBR1和Lemont中为组成型强表达。综上，YSBR1对纹枯病的高水平抗性与其防卫反应的快速启动有关。

关键词: 水稻纹枯病, 抗病种质, 蜡质, 硅, 防御反应

CLC Number:

ZUO Shimin*, ZHANG Yumei, XUE Xiang, ZHU Yajun, ZHANG Yafang, CHEN Zongxiang, CHEN Xijun, PAN Xuebiao*. Preliminary Study on Resistance Mechanism of the New Rice Line YSBR1 to Sheath Blight[J]. Chinese Journal of Rice Science, 2014, 28(2): 132-140.

左示敏* ,张玉梅,薛芗,朱亚军,张亚芳,陈宗祥,陈夕军,潘学彪*. 水稻新种质YSBR1抗纹枯病机制的初步研究[J]. 中国水稻科学, 2014, 28(2): 132-140.

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